Nanocourse: Transposon-Insertion Sequencing: the design and analysis of a new approach for bacterial genetics
Members from the Waldor and Ruben lab recently led a two-day nanocourse at Harvard Medical School on the design and analysis of transposon-insertion sequencing (TIS) experiments. The aim of the course was to provide an introduction to TIS, present experimental considerations for different types of TIS studies, and examine their respective merits and pitfalls, relevant quality controls, and impact on downstream statistical analyses. On a separate workshop day, participants also used custom scripts in Python and Matlab to carry out a full analysis of existing TIS data, including mapping raw sequence information, normalizing the data for experimental biases, and finally performing statistical analyses and generating biologically meaningful results.
Course Instructors: Dr. Michael Chao (Waldor lab), Troy Hubbard (Waldor lab), Kasia Baranowski (Ruben lab), Karen Kieser (Ruben lab) and Dr. Matthew K. Waldor.
The slides from the lectures, custom analysis scripts and data from the workshop can be downloaded here (https://drive NULL.google NULL.com/folderview?id=0Bz7imAGWYK6nfl80LUk1QnVoVnZaWThudi1zXzVVM3F2VWVmOXMwN09JOFRIU2RvNUQtd2M&usp=sharing)